Study on Isolation and Identification of Bacterium from Rhizosphere of Brinjal (Solanum melongena L.)

Abstract: Present study aimed to identify the bacteria isolated from rhizosphere of brinjal (Solanum melongena L.). The pure cultures of bacterial isolate from brinjal (Solanum melongena L.) were used to identify the bacteria. Identification of bacteria was done based on its morphological characteristics using reference strain viz. Bacillus polymyxa strain 10401 was obtained from France. Colony and morphological characters were observed under a light microscope, followed by Gram reaction and motility test. Results revealed that brinjal bacterial isolate had pearly colonies with smooth margin, constant in size, bead like and slightly raised from the surface of the culture plate. The BBI revealed rod shaped sporulating bacteria observed under light microscope. The BBI was Gram positive and turned yellow when L-Aniline 4- nitroanilide hydrochloride solution was added to the bacterial colony indicating the gram-positive nature of BBL. The bacteria were motile with the presence of single polar flagella as observed under a transmission electron microscope. The bacterial isolate which showed nitrogen fixing and phosphate solubilizing properties was identified as Bacillus polymyxa belonging to the class Eubacteriales, family Bacillaceae and genera Bacillus. In conclusion, for the first time the presence and identification of nitrogen fixing and phosphate solubilizing properties having bacteria was identified as Bacillus polymyxa on the rhizosphere of brinjal (Solanum Melongena L.).

Keyword: Morphological characteristics, Solanum Melongena L. Bacillus polymyxa, Brinjal Bacterial Isolate (BBI)