ISOLATION, MOLECULAR IDENTIFICATION AND APPLICATION OF EXOGENOUS INDOLE ACETIC ACID (IAA) PRODUCING PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) FROM SOIL

+91-7667918914 iarjset@gmail.com 0 Items International Advanced Research Journal in Science, Engineering and Technology A Monthly Peer-Reviewed Multidisciplinary Journal ISSN Online 2393-8021 ISSN Print 2394-1588 Since 2014 Home About About IARJSET Aims and Scope Editorial Board Editorial Policies Publication Ethics Publication Policies Indexing and Abstracting Citation Index License Information Authors How can I publish my paper? Instructions to Authors Benefits to Authors Why Publish in IARJSET Call for Papers Check my Paper status Publication Fee Details Publication Fee Mode FAQs Author Testimonials Reviewers Topics Peer Review Current Issue & Archives Indexing FAQ’s Contact Select Page ISOLATION, MOLECULAR IDENTIFICATION AND APPLICATION OF EXOGENOUS INDOLE ACETIC ACID (IAA) PRODUCING PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) FROM SOIL ABDUL JALEEL.R, JADHEER AHSAN K.P , SWAGATO BHATTACHARJEE, S. MOHANA PRIYA, Dr.PRAVEENA

Abstract: Plant growth promoting rhizobacteria (PGPR) are often used as inoculants to promote the growth and yield of agricultural crops, however PGPR strain selection is extremely important. This bacteria colonies plant roots and promote plant growth through a variety of mechanisms including phytohormone production, improved water and nutrient uptake, improved nitrogen availability in the soil, production of ACC-deaminase for ethylene breakdown, phosphate solubilization, siderophore production, phytoremdiation, and phytopathogen defense. Microbial Auxin production is the primary component that promotes healthy plant growth and development. The ability of PGPR to produce phytohormones can be used to boost plant growth, reducing the need for chemical fertilizers and their harmful effects on the environment. The work in this study includes isolation, screening, molecular identification, and applying effective PGPR strains based on their ability to produce auxin in vitro. The soil samples were serially diluted, and the appropriate dilution was placed onto nutrient agar media. Invitro screening was performed on isolated colonies using the Salkowski reagent, which results in a pink coloring. The existence of IAA producers is indicated by the appearance of pink colour. This isolated organism was subjected to DNA isolation after passing morphological and biochemical tests. 16S PCR was used to amplify the isolated DNA. The bacteria was identified as Pseudomonas fluorescence after sequencing and analysing the amplified products with BLAST. When compared to other strains, Pseudomonas has a higher ability to produce auxin. In addition, a pot experiment in plants was carried out to test the influence of auxin (IAA) production by the isolated strain.

Keywords: PGPR , Indole acetic acid ,BLAST Downloads: | DOI: 10.17148/IARJSET.2021.81007 How to Cite: [1] ABDUL JALEEL.R, JADHEER AHSAN K.P , SWAGATO BHATTACHARJEE, S. MOHANA PRIYA, Dr.PRAVEENA, "ISOLATION, MOLECULAR IDENTIFICATION AND APPLICATION OF EXOGENOUS INDOLE ACETIC ACID (IAA) PRODUCING PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) FROM SOIL," International Advanced Research Journal in Science, Engineering and Technology (IARJSET), DOI: IARJSET.2021.81007 Copy Citation Call for Papers Rapid Publication 24/7 April 2026 Submission: eMail paper now Notification: Immediate Publication: Immediately with eCertificates Frequency: Monthly Downloads Paper Format Copyright Form   Submit to iarjset@gmail.com or editor@iarjset.com   Submit My Paper Author CenterHow can I publish my paper? Publication Fee Why Publish in IARJSET Benefits to Authors Guidelines to Authors FAQs (Frequently Asked Questions) Author Testimonials IARJSET ManagementAims and Scope Call for Papers Editorial Board DOI and Crossref Publication Ethics Editorial Policies Publication Policies Subscription / Librarian Conference Special Issue Info ArchivesCurrent Issue & Archives Conference Special Issue Copyright © 2026 IARJSET This work is licensed under a Creative Commons Attribution 4.0 International License. Open chat