ISOLATION AND IDENTIFICATION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS [MRSA] FROM COW DUNG

Abstract: Methicillin-Resistant Staphylococcus aureus (MRSA) has been perceived as one of the significant microorganisms in both clinic and local area settings. In India, the percentage of nosocomial diseases brought about by MRSA fluctuates somewhere in the range of 20 and 40%.Conventional strategies for the identification of MRSA incorporate oxacillin disc diffusion, oxacillin MIC and oxacillin screen agar methods.In this study, Coagulase test is principally used to separate Staphylococcus aureus (positive) which produce the catalyst coagulase, from S. epidermis and S. saprophyticus (negative) which don't produce coagulase, for example Coagulase Negative Staphylococcus (CONS).For Antibiotic sensitivity test,Instead of methicillin disks,oxacillin, which has a place with a similar class (penicillinase-resistant penicillins), is preferred. These days, methicillin is neither utilized for treatment nor for testing of gram-positive microscopic organisms. Also, Oxacillin action permits a higher identification of heteroresistant strains than methicillin.

Keywords: Methicillin,Coagulase,Sensitivity