Abstract: Enzyme Alkaline Protease can be produced by microbial fermentation under optimum conditions of Temperature, pH, agitation, and aeration. In this work it is propose to use microfiltration ultrafiltration membrances as an alternative methodology to carry out fermentation process producing alkaline protease enzyme and as an alternative method for the cell separation and recovery of alkaline protease from the fermentation broth because it has the potential for energy saving and higher purity. A well- known microfiltration technique for cell separation and diafiltration for enzyme recovery is used. Ultra filtration of 20,000 MWCO is used for purification and concentration of enzyme alkaline protease.

Keywords: Biotechnology, Microbial fermentation, Membrane sterilization, Ultrafiltration.